Establishment of a Vietnamese ethnicity induced pluripotent stem cell line (VRISGi001-A) from umbilical cord blood hematopoietic stem cells under a feeder-free system


We have established the original footprint-free Vietnamese human induced pluripotent stem cell line, VRISGi001-A, from cord-blood derived CD34 + hematopoietic stem cells (HSCs) of a 35 year old healthy woman under cGMP-compliant process. For the hiPSC induction, three Sendai virus vectors carrying four reprogramming factors including c-MYC, SOX2, KLF4, and OCT3/4 were delivered into CD34+ HSCs. The VRISGi001-A cell line expresses the majority of the pluripotent markers and differentiate in vitro into derivatives of three germ layers. The availability of Vietnamese hiPSC line could contribute to the improvement of inadequate genetic diversity in the currently available hiPSC lines. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Tran TT, Nguyen TH, Nguyen TT, Nguyen XH
Journal Stem cell research
Publication Date 2021 May;53:102345
PubMed 33878709
DOI 10.1016/j.scr.2021.102345

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