CS0791iCTR-LBCn1

General#

Cell Line

hPSCreg Name EDi023-A
Alternative name(s)
CS0791iCTR-LBCn1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 30th January 2019
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Provider

Generator University of Edinburgh (ED)

External Databases

BioSamples SAMEA5274199
Cellosaurus CVCL_VT09

General Information

Projects
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA5274200

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection EBNA negative

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Self-renewal
Unkown
Endoderm
Unkown
Mesoderm
Unkown
Ectoderm score
Positive

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 10

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal female Karyotype
Karyotyping method: G-Banding

Other Genotyping (Cell Line)