General

Cell Line
hPSCreg name	RGIe053-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	RGIe053-A (RRID:CVCL_B568)
Alternative name(s)	SI-141
Cell line type	Human embryonic stem cell (hESC)
Similar lines	No similar lines found.
Last update	4th September 2022
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Provider
Generator	Reproductive Genetics Institute (RGI) Contact: Stem Cell Research Unit
Derivation country	United States
External Databases
Cellosaurus	CVCL_B568
Wikidata	Q54949891
General Information
Publications	Verlinsky Y et al. Human embryonic stem cell lines with genetic disorders. Reproductive biomedicine online. 2005 Jan;10(1):105-10.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Neurofibromatosis type 1 The donor is affected. Synonyms neurofibromatosis NF1 Nf1-Microdeletion syndrome neurofibromatosis 1 neurofibromatosis type 1 neurofibromatosis, type 1 peripheral neurofibromatosis Recklinghausen's disease Von Recklinghausen disease neurofibromatosis type 1 microdeletion syndrome neurofibromatosis, peripheral type neurofibromatosis, type I type 1 neurofibromatosis von Reklinghausen disease neurofibromatosis type i Neurofibromatosis 1 nonmosaic NF1 nonmosaic neurofibromatosis type 1 show more synonyms show less synonyms
Disease associated phenotypes	Type I affected

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	Yes

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
POU5F1 (OCT-4)	Yes
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
TRA 2-39	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes
Other Genotyping (Cell Line)