AdiPS 1
CAMi004-A
General
Cell Line |
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| hPSCreg name | CAMi004-A |
| Cite as: | CAMi004-A (RRID:CVCL_2Z93) |
| Alternative name(s) |
AdiPS 1
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 19th August 2019 |
| User feedback | |
Provider |
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| Generator | University of Cambridge (CAM) |
External Databases |
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| Cellosaurus | CVCL_2Z93 |
| Wikidata | Q54808440 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 50-54 |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Contact information / weblink | |
| Alternatives to consent | |
| Alternative consent approval number | |
| Has the donor been informed about how her/his data will be protected? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Details on restriction to research project | |
| How may genetic information associated with the cell line be accessed? | |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Please describe how access is provided: | |
| Contact data, institution, or website: | |
| Name of accrediting authority involved? | |
| Approval number | |
| Name of accrediting authority involved? | |
| Approval number | |
| Please describe: | |
| Further constraints on use | |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Constraints for use or distribution |
hIPSC Derivation
General |
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| Source cell origin |
Portion of connective tissue composed of adipocytes enmeshed in areolar tissue.
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| Age of donor (at collection) | 50-54 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | ||||||||||||||||
| Feeder cells |
Yes |
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| Passage method |
Enzymatically
Collagenase
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| Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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