KCL009_trans1

General#

Cell Line

hPSCreg Name KCLe008-A
Alternative name(s)
KCL009_trans1
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A244

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
chromosomal disease
The donor is affected.
Disease associated phenotypes
  • chromosomal abnormalities

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent

hESC Derivation#

PGD Embryo?
Yes

Culture Conditions#

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX,der(7)t(7;12)(q31.1;p13.1)pat

Other Genotyping (Cell Line)