UPSFRe003-A

CL03

General

Cell Line

hPSCreg Name
UPSFRe003-A
Alternative name(s)
CL03
Cell line type Human embryonic stem cell (hESC)
Last update 30th August 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Université Paris-Sud 11 (UPSFR)
Contact:
ESTeam Paris Sud
Derivation country France

External Databases

Cellosaurus CVCL_C798
CLO CLO_0102639
Wikidata Q54813495

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
chromosomal disease
The donor is affected.
Disease associated phenotypes
  • chromosomal abnormalities, Balanced translocation

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Agence de la biomédecine
Approval number

hESC Derivation

Date of derivation 2009-07-21
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
Expansion status 6
Stephenson 2007
ICM morphology A
Stephenson 2007
Trophectoderm morphology a
Stephenson 2007
ZP removal technique Spontaneous Hatching
Cell isolation None
Cell seeding Embryo

Culture Conditions

Feeder cells Mouse Embryonic Fibroblast
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
2-mercaptoetanol
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
CD9
Yes
GABRB3
Yes
LEFTB
Yes
LIN28
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TDGF1
Yes
SSEA-4
Yes
TRA 1-60
Yes
HESCA-1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY,t(8;9)(p23;p24).ish t(8;9)(8pter-,9pter+,8qter+;9pter-,8pter+,9qter+)
Passage number: 13

Other Genotyping (Cell Line)