RH 5
EDe004-A
General
Cell Line |
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| hPSCreg name | EDe004-A |
| Cite as: | EDe004-A (RRID:CVCL_C358) |
| Alternative name(s) |
RH 5
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
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| Last update | 22nd March 2021 |
| User feedback | |
Provider |
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| Generator | University of Edinburgh (ED) |
| Derivation country | United Kingdom |
External Databases |
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| Cellosaurus | CVCL_C358 |
| Wikidata | Q54950363 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes |
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Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Supernumerary embryos from IVF treatment? |
Yes
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| PGD Embryo? |
No |
Culture Conditions
| Surface coating | Gelatin | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
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| TRA 1-60 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
Passage number: 33
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Other Genotyping (Cell Line) |
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