Generation of a gene corrected human isogenic IBMS-iPSC-014-C from polycystic-kidney-disease induced pluripotent stem cell line using CRISPR/Cas9


We report the engendering an isogenic iPSC line from the IBMS-iPSC-014-05 with homozygous correction of the R803X, Chr4: 88989098C > T in PKD2, using CRISPR/Cas9 technology. The results from the isogenic control, IBMS-iPSC-014-05C, showed that mutation had been corrected, while maintaining normal morphology, pluripotency, and differentiation capacity into three germ layers. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Liu CL, Huang CY, Chen HC, Lu HE, Hsieh PCH, Lee JJ
Journal Stem cell research
Publication Date 2020 May;45:101784
PubMed 32361310
DOI 10.1016/j.scr.2020.101784

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