Generation of a gene corrected human isogenic IBMS-iPSC-014-C from polycystic-kidney-disease induced pluripotent stem cell line using CRISPR/Cas9
Summary
We report the engendering an isogenic iPSC line from the IBMS-iPSC-014-05 with homozygous correction of the R803X, Chr4: 88989098C > T in PKD2, using CRISPR/Cas9 technology. The results from the isogenic control, IBMS-iPSC-014-05C, showed that mutation had been corrected, while maintaining normal morphology, pluripotency, and differentiation capacity into three germ layers. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Liu CL, Huang CY, Chen HC, Lu HE, Hsieh PCH, Lee JJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 May;45:101784 |
| PubMed | 32361310 |
| DOI | 10.1016/j.scr.2020.101784 |