Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)
Summary
Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN technology. The reporter enables the optimization and monitoring of hiPSC differentiation towards NKX2.1/p63 double positive cells as well as enrichment for single or double positive cells. Copyright © 2019. Published by Elsevier B.V.
| Authors | Drick N, Sahabian A, Pongpamorn P, Merkert S, Göhring G, Welte T, Martin U, Olmer R |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Jan;42:101659 |
| PubMed | 31791010 |
| DOI | 10.1016/j.scr.2019.101659 |