Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)
Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN technology. The reporter enables the optimization and monitoring of hiPSC differentiation towards NKX2.1/p63 double positive cells as well as enrichment for single or double positive cells. Copyright © 2019. Published by Elsevier B.V.
|Authors||Drick N, Sahabian A, Pongpamorn P, Merkert S, Göhring G, Welte T, Martin U, Olmer R|
|Journal||Stem cell research|
|Publication Date||2020 Jan;42:101659|