Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)

Summary

Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN technology. The reporter enables the optimization and monitoring of hiPSC differentiation towards NKX2.1/p63 double positive cells as well as enrichment for single or double positive cells. Copyright © 2019. Published by Elsevier B.V.

Authors Drick N, Sahabian A, Pongpamorn P, Merkert S, Göhring G, Welte T, Martin U, Olmer R
Journal Stem cell research
Publication Date 2019 Nov 19;42:101659
PubMed 31791010
DOI 10.1016/j.scr.2019.101659

Research Projects

Cell Lines