HES-1
ESIBIe001-A
General
Cell Line |
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| hPSCreg name | ESIBIe001-A |
| Cite as: | ESIBIe001-A (RRID:CVCL_D092) |
| Alternative name(s) |
HES-1
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 13th September 2017 |
| User feedback | |
Provider |
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| Generator |
ES Cell International Pte Ltd. (ESIBI)
Contact:
hES cell provision |
| Derivation country | Singapore |
External Databases |
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| Cellosaurus | CVCL_D092 |
| Wikidata | Q54882904 |
General Information |
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| Publications |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
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| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Enzymatically and Mechanically | ||||||
| Medium |
Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| TDGF1 |
Yes |
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| SSEA-3 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 46
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Other Genotyping (Cell Line) |
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