SNUhES11
SNUe005-A
General
Cell Line |
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| hPSCreg name | SNUe005-A |
| Cite as: | SNUe005-A (RRID:CVCL_9946) |
| Alternative name(s) |
SNUhES11
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
Seoul National University (SNU)
Contact:
Stem Cell Biology |
| Derivation country | South Korea |
External Databases |
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| Cellosaurus | CVCL_9946 |
| Wikidata | Q54955313 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclones | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
No
Other hESC source:
IVF
|
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | Gelatin | |||||||||
| Feeder cells |
mouse embryonic fibroblast cells Cellfinder Ont Id: EFO_0004040 |
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| Passage method | Mechanically | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 26
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Other Genotyping (Cell Line) |
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