SNUhES11

General

Cell Line

hPSCreg Name
SNUe005-A
Alternative name(s)
SNUhES11
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Seoul National University (SNU)
Derivation country South Korea

External Databases

Cellosaurus CVCL_9946
CLO CLO_0100470
Wikidata Q54955313

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
No
Other hESC source: IVF
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Surface coating Gelatin
Feeder cells mouse embryonic fibroblast cells
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 26

Other Genotyping (Cell Line)