SNUe005-A-1

General#

Cell Line

hPSCreg Name SNUe005-A-1
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Seoul National University (SNU)
Derivation country South Korea

External Databases

CLO CLO_0102886

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Also have a look at the ethics information for the parental line SNUe005-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells STO Cell line
Cellfinder Ont Id: CLO_0009149
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XY,+12
Passage number: 26

Other Genotyping (Cell Line)

Genetic Modification#