SNUe005-A-1

General

Cell Line

hPSCreg Name
SNUe005-A-1
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator Seoul National University (SNU)
Derivation country South Korea

External Databases

CLO CLO_0102886

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics

Also have a look at the ethics information for the parental line SNUe005-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line SNUe005-A.

Culture Conditions

Feeder cells STO Cell line
Cellfinder Ont Id: CLO_0009149
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XY,+12
Passage number: 26

Other Genotyping (Cell Line)

Genetic Modification