SNUe005-A-1
General
iPSC Line |
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| hPSCreg name | SNUe005-A-1 |
| Cite as: | SNUe005-A-1 |
| iPSC line type | Human embryonic stem cell (hESC) |
| Similar iPSC lines |
SNUe005-A (SNUhES11) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
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| Generator |
Seoul National University (SNU)
Contact:
Stem Cell Biology |
| Derivation country | South Korea |
External iPSC Databases |
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General iPSC Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
iPSC Genetic Modification
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
Also have a look at the ethics information for the parental line
SNUe005-A
.
| Alternatives to consent | |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
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The source cell information can be found in the parental cell line
SNUe005-A.
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iPSC Culture Conditions
| Feeder cells |
STO Cell line Cellfinder Ont Id: CLO_0009149 |
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| Passage method | Mechanically | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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iPSC Characterisation
Analysis of undifferentiated iPSCs
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
47XY,+12
Passage number: 26
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Other Genotyping (iPSC Line) |
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