ITXi002-A-3

IRX5-KI, CRISPR-3 IRX5 F6 KI

General

Cell Line

hPSCreg Name
ITXi002-A-3
Alternative name(s)
IRX5-KI, CRISPR-3 IRX5 F6 KI
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 2nd February 2022
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Provider

Generator l’institut du thorax (ITX)

External Databases

BioSamples SAMEA11025522
CLO CLO_0103724
Cellosaurus CVCL_B5QG
Wikidata Q111733530

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
No major chromosomal aberrations were found

External Databases (Donor)

BioSamples SAMEA13166857

Ethics

Also have a look at the ethics information for the parental line ITXi002-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line ITXi002-A.

Reprogramming method

Vector type None

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
Ectoderm
Ont Id: NCIT_C12703
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array

Genetic Modification

Genetic modifications not related to a disease
IRX5 (target)
Gene knock-in
Transgene: HA-tag sequence
CRISPR-associated (CRISPR/Cas) System