Generation of human induced pluripotent stem cell line (BCRTi007-A) from urinary cells of a patient with autosomal dominant polycystic kidney disease


Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disorder of adults, characterized by uncontrolled cysts formation that causes a gradual impairment of kidney function. We generated a human induced pluripotent stem cell (hiPSC) line from the urinary cells of a patient diagnosed with ADPKD using a non-integrating Epi5™ Episomal iPSC reprogramming strategy. Characterization of the cell line was performed regarding their undifferentiated status, differentiation potential, and quality control for karyotypic integrity, identity, and clearance of reprogramming vectors. The newly derived hiPSC line, namely BCRTi007-A, can be used in vitro for disease modeling of ADPKD as well as testing for novel therapeutic approaches. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Batool L, Raab C, Beez CM, Kurtz A, Gollasch M, Rossbach B
Journal Stem cell research
Publication Date 2023 Jun;69:103071
PubMed 36947994
DOI 10.1016/j.scr.2023.103071

Research Projects

Cell Lines