Generation of human iPSCs from fetal prostate fibroblasts HPrF


Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Kahounová Z, Slabáková E, Binó L, Remšík J, Fedr R, Bouchal J, Vrtěl R, Jurečková L, Porokh V, Páralová D, Hampl A, Souček K
Journal Stem cell research
Publication Date 2019 Mar;35:101405
PubMed 30776675
DOI 10.1016/j.scr.2019.101405

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