Generation of human iPSCs from fetal prostate fibroblasts HPrF
Summary
Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Kahounová Z, Slabáková E, Binó L, Remšík J, Fedr R, Bouchal J, Vrtěl R, Jurečková L, Porokh V, Páralová D, Hampl A, Souček K |
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| Journal | Stem cell research |
| Publication Date | 2019 Mar;35:101405 |
| PubMed | 30776675 |
| DOI | 10.1016/j.scr.2019.101405 |