IPS35-2

General

Cell Line

hPSCreg name AHQUi001-A
Cite as:
AHQUi001-A (RRID:CVCL_YK97)
Alternative name(s)
IPS35-2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
AHQUi001-A-1
(IPS35-2-R)
Donor's gene variants:
LPL
Donor diseases:
NON RARE IN EUROPE: Hyperlipoproteinemia type 4
SCVIi109-A
(SCVI3020)
Donor diseases:
familial hypercholesterolemia
SCVIi108-A
(SCVI2767)
Donor diseases:
familial hypercholesterolemia
PNUSCRi003-A
(GBA Gaucher iPSC1)
Donor diseases:
Gaucher disease
SDQLCHi030-A
Donor's gene variants:
HPRT1
Donor diseases:
Lesch-Nyhan Syndrome
CMCi006-A
(CMC-Fb-001)
Donor diseases:
Fabry Disease
NJDTHi001-A
Donor diseases:
Danon disease
Last update 29th November 2021
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Provider

Generator Affiliated Hospital of Qingdao University (AHQU)
Derivation country China

External Databases

BioSamples SAMEA6516971
Cellosaurus CVCL_YK97
Wikidata Q93323833

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information

Sex male
Ethnicity Chinese Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Familial hypertriglyceridemia (FHTG)
The donor is a carrier of a disease-associated mutation.
Synonyms
  • NON RARE IN EUROPE: Familial hypertriglyceridemia
  • NON RARE IN EUROPE: HLP type 4
Genetic variants
LPL (target)
NM_000237.3:c.928 T>C
p.C310R
Heterozygous

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6516985

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Xiaofang Sun (Email: xiaofang_3399@163.com)
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Xiaofang Sun (Email: xiaofang_3399@163.com)
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the ethics committee of the Affiliated Hospital of Qingdao University
Approval number QYFY WZLL 25646
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? the ethics committee of the Affiliated Hospital of Qingdao University
Approval number QYFY WZLL 25646
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
Immunostaining, RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
TRA 1-60
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)