Establishment of a CRISPR/Cas9-mediated GATAD2B homozygous knockout human embryonic stem cell line


As a subunit of the nucleosome remodeling and histone deacetylation (NuRD) complex, GATA zinc finger domain containing 2B (GATAD2B) plays a vital role in chromatin modification and transcriptional regulation. To further investigate the role of GATAD2B in cell fate determination of human ESCs, we generated two GATAD2B homozygous knockout human ESC lines by CRISPR/Cas9 technology. The cell line exhibits normal karyotype and typical stem cell morphology, following the high expression of pluripotent genes and differentiation potential in vitro. These cell lines will provide cell resources to investigate epigenetic regulation in extensive biological processes as menthioned above. Copyright © 2021. Published by Elsevier B.V.

Authors Wang B, Zhu S, Deng Y, Sai X, Chen Z, Liu J, Li G, Liu N, Chen J, Yu C, Sun T, Zhu P
Journal Stem cell research
Publication Date 2021 Dec;57:102590
PubMed 34749018
DOI 10.1016/j.scr.2021.102590

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