ICGi024-A
General
Donor Information
General Donor Information |
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Sex | male |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
46,XY,r(18)(p11.1q23)[38]. arr[hg19] 18p11.32p11.21(14316_13530125)×1 dn, 18q23(76744252_77982126)×1 dn
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA7332542 |
Ethics
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
Has the donor agreed to be re-contacted? | Unknown |
Has the donor been informed about how her/his data will be protected? | Yes |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
How may genetic information associated with the cell line be accessed? | Controlled Access |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | The study was approved by the Scientific Ethics Committee of Research Institute of Medical Genetics, Tomsk NRMC |
Approval number | protocol number 106/2017 |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
Name of accrediting authority involved? | The study was approved by the Scientific Ethics Committee of Research Institute of Medical Genetics, Tomsk NRMC |
Approval number | protocol number 106/2017 |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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Source cell type | |
Passage number reprogrammed | 4 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector map | |
Vector free reprogramming |
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Other |
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Selection criteria for clones | morphology marker expression |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Gelatin | ||||||||||||||||||
Feeder cells |
mouse embryonic fibroblasts Cellfinder Ont Id: EFO_0004040 |
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Passage method | Mechanically | ||||||||||||||||||
CO2 Concentration | 5 % | ||||||||||||||||||
Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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TRA 1-60 |
Yes |
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SOX2 |
Yes |
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SSEA-4 |
Yes |
Marker | Present | Absent |
mCpG | ||
OCT4 |
Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
Protocol or reference
In vitro spontaneous differentiation
Protocol or reference
In vitro spontaneous differentiation
Protocol or reference
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46,XY,r(18)[50]/45,XY,-18[6]/47,XY,r(18),r(18)[1]
Passage number: 6
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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