Establishment of SIAISi017-A, an induced pluripotent stem cell(iPSC)line from a healthy 25-year-old Chinese Hui
Summary
With the development of cytology, the establishment of cell models in vitro has become a powerful means to study the mechanism and treatment of diseases. Here we successfully generated the IPSC-derived modeling system of a 25-year-old healthy male. His peripheral blood mononuclear cells (PBMC) were reprogrammed using human OKSM (SOX2, OCT3/4, KLF4, and C-MYC) transcription factors using a non-integrated additional vector system. Immunocytochemistry demonstrated that IPSCS expressed all the markers of pluripotency and demonstrated their ability to differentiate spontaneously from three hypoderms in vitro. Karyotype is normal. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Zhang Y, Shou J, Gu W, Di W, Zhao J, Wang Y, Gu S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Apr;60:102645 |
| PubMed | 35093714 |
| DOI | 10.1016/j.scr.2021.102645 |