K10, hFF-iPSCs

General#

Cell Line

hPSCreg Name
MILi001-A
Alternative name(s)
K10, hFF-iPSCs
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 2nd June 2021
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Provider

Generator Miltenyi Biotec B.V. & Co. KG (MIL)
Owner Miltenyi Biotec B.V. & Co. KG (MIL)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA9086589
CLO CLO_0103395

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history n.a.
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,9 D16S539: 9,13 D5S818: 12 D7S820: 11,12 TH01: 7,8 TPOX: 10,11 vWA: 16,18

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5176968

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? No
Was the consent voluntarily given? No
Has the donor been informed that participation will not directly influence their personal treatment? No
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: https://www.atcc.org/en/support/technical-support
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Alternatives to consent are available? Yes
Alternatives to consent MTA
Alternative consent approval number
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: as outlined in MTA
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Miltenyi Biotec B.V. & Co. KG
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution only to be distributed upon approval of ATCC according to MTA

hIPSC Derivation#

General

Source cell line name BJ
Other names
Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
Source cell origin
Foreskin
Source cell type (free text) ATCC® CRL-2522
Source cell line vendor ATCC
Passage number reprogrammed n.a

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Selection criteria for clones Expression of TRA-1-60, immunomagnetic selection MACS, subcultivation, morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Laminin
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS iPS-Brew XF
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
day 5 of cultivation / TrypLE single cell splitting
day 5 cultivation / StemMACS Passaging solution (cluster passaging)
StemMACS™ Trilineage Differentiation Kit, human
Method documentation
StemMACS™ Trilineage.pdf
User Manual StemMACS Trilineage Differentiation Kit, human
Flow cytometric analysis_Trilineage Kit.pdf
Application Note Tri Lineage Assay
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Other
Morphology
Teratoma Endoderm
K10 Endoderm ICC_CXCR4 rot_Sox17 grün_Draq5.tif
red: CXCR4; green: Sox17; blue: DRAQ5
directed differentiation - StemMACS Trilineage Kit
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro directed differentiation
Marker Expressed
PDGFRB
Yes
Morphology
directed differentiation with StemMACS Trilineage assay: CD144 (enothelial cells) / CD140b = PDGFRB smooth muscle cells
directed differentiation with StemMACS Trilineage assay: CD144 (enothelial cells) / CD140b = PDGFRB smooth muscle cells (red); endothelial cells (CD144) green
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Morphology
Teratoma: cartilage
Endothelial cells
In vitro directed differentiation
Morphology
directed differentiation with StemMACS Trilineage assay: CD144 (enothelial cells) / CD140b = PDGFRB smooth muscle cells
directed differentiation with StemMACS Trilineage assay: CD144 (enothelial cells) / CD140b = PDGFRB smooth muscle cells (red); endothelial cells (CD144) green
Neuroepithelial Stem Cell
Ont Id: CL_0002259
In vivo teratoma
In vitro directed differentiation
Marker Expressed
PAX6
Yes
SOX2
Yes
Morphology
K10 Ectoderm_ICC_Pax6 rot_Sox2 grün_ Draq5.tif
directed differentiation with StemMACS Trilineage assay: red = PAX6; green = SOX2; blue=DRAQ5
directed differentiation with StemMACS Trilineage assay: FC PAX6/SOX2

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY
Passage number: p19
Karyotyping method: G-Banding

Other Genotyping (Cell Line)