TUR1
UTUi001-A
General
Cell Line |
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| hPSCreg name | UTUi001-A |
| Cite as: | UTUi001-A (RRID:CVCL_D0PB) |
| Alternative name(s) |
TUR1
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 4th January 2024 |
| User feedback | |
Provider |
|
| Generator |
University of Turku (UTU)
Contact:
University of Turku (UTU) |
| Owner | University of Turku (UTU) |
| Distributors | |
| Derivation country | Finland |
External Databases |
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| BioSamples | SAMEA112302760 |
| Cellosaurus | CVCL_D0PB |
| Wikidata | Q123033764 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information
General Donor Information |
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| Sex | male |
| Age of donor (at collection) | neonate |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | No |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46, XY
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA5574031 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | This iPS cell line was derived from the BJ Fibroblast cell lines that are readily available through ATCC. The holder of the original information is the American Type Culture Collection (ATCC), 10801 University Boulevard. Manassas, VA 20110 USA. |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | http://www.lgcstandards-atcc.org/products/all/CRL-2522.aspx#characteristics |
| Has the donor agreed to be re-contacted? | Unknown |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethics Committee for Human Sciences at the University of Turku |
| Approval number | 6/2023 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell line name |
BJ Other names
Derived from same source line (potentially other lot and donor, see below):
|
| Source cell type |
Synonyms
|
| Source cell origin | |
| Source cell type (free text) | ATCC® CRL-2522 |
| Age of donor (at collection) | neonate |
| Collected in | 1986 |
| Source cell line vendor | ATCC |
| Passage number reprogrammed | 7 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Is reprogramming vector detectable? |
Yes |
| Methods used |
PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Selection criteria for clones | Morphology of iPS colonies |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-4 |
Yes |
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| SSEA-1 |
No |
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Morphology pictures
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| SOX17 |
Yes |
| LEFTY1 |
Yes |
Morphology
In vitro spontaneous differentiation
| Marker | Expressed |
| Brachyury |
Yes |
| HAND1 |
Yes |
Morphology
In vitro spontaneous differentiation
| Marker | Expressed |
| PAX6 |
Yes |
| TUBB3 |
Yes |
Morphology
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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