KUIFMSi011-A

246G1

General#

Cell Line

hPSCreg Name KUIFMSi011-A
Alternative name(s)
246G1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_C243

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

Other cell lines of this donor

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name BJ
Other names
Derived from same source line (potentially other lot and donor, see below):
Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) neonate

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells SNL76/7
Cellfinder Ont Id: CELDA_00013400
Passage method Mechanically
Medium Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source:
Serum concentration: %
Supplements
hrbFGF 4 ng/ml %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GDF3
Yes
ZFP42 (REX-1)
Yes
POU5F1
Yes
SOX2
Yes
NANOG
Yes
FGF4
Yes
Dppa5
Yes
DPPA4
Yes
DPPA2
Yes
TERT
Yes
KLF4
Yes
MYC
Yes
NAT1
Yes

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)