KUIFMSi011-A

Cell Line
hPSCreg name	KUIFMSi011-A
Cite as:	KUIFMSi011-A (RRID:CVCL_C243)
Alternative name(s)	246G1
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	KUIFMSi011-B (246G3) KUIFMSi011-C (246G4) KUIFMSi011-D (246G5) KUIFMSi011-E (246G6)
Last update	3rd September 2022
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Provider
Generator	Kyoto University (KUIMS) Contact: iPS Cell Research and Application
Derivation country	Japan
External Databases
Cellosaurus	CVCL_C243
Wikidata	Q54583529
General Information
Publications	Takahashi K et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30;131(5):861-72. Koyanagi-Aoi M et al. Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells. Proceedings of the National Academy of Sciences of the United States of America. 2013 Dec 17;110(51):20569-74. Nishizawa M et al. Epigenetic Variation between Human Induced Pluripotent Stem Cell Lines Is an Indicator of Differentiation Capacity. Cell stem cell. 2016 Sep 1;19(3):341-54.
* Is the cell line readily obtainable for third parties?	No

General Donor Information
Sex	male
Age of donor (at collection)	neonate
Phenotype and Disease related information (Donor)
Diseases
Donor Relations
Other cell lines of this donor	KUIFMSi011-B KUIFMSi011-C KUIFMSi011-D KUIFMSi011-E

How may genetic information associated with the cell line be accessed?
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

General
Source cell line name	BJ Other names CVCL_3653 in Cellosaurus CRL-2522 in ATCC Derived from same source line (potentially other lot and donor, see below): KUIFMSi011-B KUIFMSi011-C KUIFMSi011-D KUIFMSi011-E RCSIi001-A HVRDi005-A REGUi001-A REGUi002-A UNIPDi004-A UNIPDi004-B CRTDi005-A CRTDi005-B ISFi001-A BGUi015-A MILi001-A ISFi002-A MILi001-B MILi001-C HHUUKDi009-A UTUi001-A UTUi001-B UTUi001-C UNIPDi004-D
Source cell type	Fibroblast Synonyms fibroblast Fibroblasts Fibroblast FIBROBLAST show more synonyms show less synonyms
Source cell origin	Foreskin
Source cell type (free text)	ATCC® CRL-2522
Age of donor (at collection)	neonate
Reprogramming method
Vector type	Integrating
Vector	Virus (Lentivirus)
Genes	POU5F1 SOX2 KLF4 MYC
Is the used vector excisable?	Unknown
Absence of reprogramming vector(s)?	Unknown
Reprogramming vectors silenced?
Vector free reprogramming
Type of used vector free reprogramming factor(s)	None
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Surface coating

Matrigel/Geltrex

Feeder cells

SNL76/7
Cellfinder Ont Id: CELDA_00013400

Passage method

Mechanically

Medium

Other medium:

Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source:
Serum concentration: %

Supplements

Supplement	Amount	Unit
hrbFGF	4 ng/ml	%

Marker Expression

Karyotyping (Cell Line)
Other Genotyping (Cell Line)

246G1