BJ SiPS-D
HVRDi005-A
General
Cell Line |
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| hPSCreg name | HVRDi005-A |
| Cite as: | HVRDi005-A (RRID:CVCL_X741) |
| Alternative name(s) |
BJ SiPS-D
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 2nd March 2021 |
| User feedback | |
Provider |
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| Generator | Harvard University (HVRD) |
External Databases |
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| Cellosaurus | CVCL_X741 |
| BioSamples | SAMEA8325233 |
| Wikidata | Q54796923 |
General Information |
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| Publications |
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| Projects | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
| Family history | n.a. |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
SNP typing array
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,9 D16S539: 9,13 D5S818: 12 D7S820: 11,12 TH01: 7,8 TPOX: 10,11 vWA: 16,18 |
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA5176968 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | No |
| Was the consent voluntarily given? | No |
| Has the donor been informed that participation will not directly influence their personal treatment? | No |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | This iPS cell line was derived from the BJ Fibroblast cell lines that are readily available through ATCC. As far as we know there was no consent. The derivation was considered Not Human Subjects Research. I am not answering some of the questions below because there is no consent so the questions are not applicable. |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | There is no consent available for these lines because they were derived from the BJ fibroblast cell line distributed by ATCC and the derivation was Not Human Subjects Research. Attached is the letter that the iPS Core Repository received when the lines were deposited into the repository for distribution. The lines are not currently distributed from the repository. |
| Alternative consent approval number | N/A |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
| Do you have obligations to third parties in regard to the use of the cell line? | Yes |
| Please describe: | see MTA |
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell line name |
BJ Other names
Derived from same source line (potentially other lot and donor, see below):
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| Source cell type |
Synonyms
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| Source cell origin | |
| Source cell type (free text) | ATCC® CRL-2522 |
| Source cell line vendor | ATCC |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemFlex
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| SSEA-4 |
Yes |
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| SSEA-3 |
Yes |
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| TRA 1-60 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| AFP |
Yes |
| GATA4 |
Yes |
Morphology
BJ-SiPS 3 germ layer markers.tif
PCR for markers of the three germ layers
BJ-SiPS pluripotency markers.tif
Pluripotency markers
In vitro spontaneous differentiation
| Marker | Expressed |
| FLK1 |
Yes |
| VECAD |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| Pax6 |
Yes |
| N-CAM |
Yes |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY, Normal Human Male Karyotype
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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