246G5	
    			    			
                KUIFMSi011-D            
            
        General
| Cell Line | |
| hPSCreg name | KUIFMSi011-D | 
| Cite as: | KUIFMSi011-D (RRID:CVCL_C246) | 
| Alternative name(s) | 
	246G5	 | 
| Cell line type | Human induced pluripotent stem cell (hiPSC) | 
| Similar lines | |
| Last update | 3rd September 2022 | 
| User feedback | |
| Provider | |
| Generator | Kyoto University (KUIMS) 
    									Contact:
    									 iPS Cell Research and Application | 
| Derivation country | Japan | 
| External Databases | |
| Cellosaurus | CVCL_C246 | 
| Wikidata | Q54583536 | 
| General Information | |
| Publications | |
| * Is the cell line readily obtainable for third parties? | No | 
Donor Information
| General Donor Information | |
| Sex | male | 
| Age of donor (at collection) | neonate | 
| Phenotype and Disease related information (Donor) | |
| Diseases | |
| Donor Relations | |
| Other cell lines of this donor | |
Ethics
| How may genetic information associated with the cell line be accessed? | |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | 
hIPSC Derivation
| General | |
| Source cell line name | BJ Other names Derived from same source line (potentially other lot and donor, see below):
							
							 | 
| Source cell type | Synonyms 
 | 
| Source cell origin | |
| Source cell type (free text) | ATCC® CRL-2522 | 
| Age of donor (at collection) | neonate | 
| Reprogramming method | |
| Vector type | Integrating | 
| Vector | Virus (Lentivirus) | 
| Genes | |
| Is the used vector excisable? | Unknown | 
| Absence of reprogramming vector(s)? | Unknown | 
| Reprogramming vectors silenced? | |
| Vector free reprogramming | |
| Type of used vector free reprogramming factor(s) | 
	None	 | 
| Other | |
| Derived under xeno-free conditions | Unknown | 
| Derived under GMP? | Unknown | 
| Available as clinical grade? | Unknown | 
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells | SNL76/7 Cellfinder Ont Id: CELDA_00013400 | ||||||
| Passage method | Mechanically | ||||||
| Medium | Other medium: 
			Base medium: Primate ES medium (ReproCELL, Japan)			 Main protein source: Serum concentration: % Supplements 
 | 
Characterisation
Genotyping
| Karyotyping (Cell Line) | |
| Other Genotyping (Cell Line) | 

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