IPS4FLV

General#

Cell Line

hPSCreg Name REGUi001-A
Alternative name(s)
IPS4FLV
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 18th March 2019
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Provider

Generator Institute for Regenerative Medecine & Biotherapy U1183 (REGU)
Owner Institute for Regenerative Medecine & Biotherapy U1183 (REGU)
Distributors
Derivation country France

External Databases

BioSamples SAMEA5574030

General Information

Projects
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5574031

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name BJ
Other names
Derived from same source line (potentially other lot and donor, see below):
Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell line lot number
lot 2
Age of donor (at collection) neonate
Collected in 2000
Source cell line vendor ATCC
Passage number reprogrammed 50

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones morphology of iPSC clones
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unkown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unkown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Unkown

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
Transcriptome Characterisation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Cell Of Skeletal Muscle
Ont Id: CL_0000188
In vivo teratoma

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)