KUIFMSi011-C

246G4

General#

Cell Line

hPSCreg Name KUIFMSi011-C
Alternative name(s)
246G4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator Kyoto University (KUIMS)
Derivation country Japan

External Databases

Cellosaurus CVCL_C245

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) neonate

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

Other cell lines of this donor

Ethics#

Provide contact information of the holder of the original Donor Information Sheet:
Contact information / weblink
Alternatives to consent
Alternative consent approval number
Details on restriction to research project
How may genetic information associated with the cell line be accessed?
Please describe how access is provided:
Contact data, institution, or website:
Name of accrediting authority involved?
Approval number
Name of accrediting authority involved?
Approval number
Please describe:
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation#

General

Source cell line name BJ
Other names
Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
Foreskin
Age of donor (at collection) neonate

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells SNL76/7
Cellfinder Ont Id: CELDA_00013400
Passage method Mechanically
Medium Other medium:
Base medium: Primate ES medium (ReproCELL, Japan)
Main protein source:
Serum concentration: %
Supplements
hrbFGF 4 ng/ml %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GDF3
Yes
ZFP42 (REX-1)
Yes
POU5F1
Yes
SOX2
Yes
NANOG
Yes
FGF4
Yes
Dppa5
Yes
DPPA4
Yes
DPPA2
Yes
TERT
Yes
KLF4
Yes
MYC
Yes
NAT1
Yes

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)