Generation and characterization of GATA6-specific EGFP expressing human induced pluripotent stem cell line, KSCBi017-A-1, using CRISPR/Cas9
Summary
GATA6 is expressed during early embryogenesis and localizes to endoderm- and mesoderm-derived tissues during later embryogenesis. Here, we established a human induced pluripotent stem cell (hiPSC) line expressing EGFP under GATA6 gene. EGFP coding sequence was introduced into the C-terminus of GATA6 in KSCBi017-A hiPSCs through homologous recombination using CRISPR/Cas9 system. The successfully edited line, KSCBi017-A-1, was selected and confirmed by sequencing. The line had a normal karyotype and exhibited potential to differentiate into three germ layers while it expressed EGFP upon endoderm induction. KSCBi017-A-1 cells can be used to monitor the expression of GATA6 during differentiation. This cell line is available from Korea National Stem Cell Bank. Copyright © 2024. Published by Elsevier B.V.
| Authors | Bayarsaikhan D, Yoo DH, Lee J, Im YS, Bayarsaikhan G, Kang HA, Kim YO, Lee B |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr 24;77:103426 |
| PubMed | 38678980 |
| DOI | 10.1016/j.scr.2024.103426 |