KSCBi017-A-1

PB01-EiPS21_GATA6-EGFP

The cell line is not validated yet.

General

Cell Line

hPSCreg name KSCBi017-A-1
Cite as:
KSCBi017-A-1 (RRID:CVCL_D6MJ)
Alternative name(s)
PB01-EiPS21_GATA6-EGFP
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 17th July 2025
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Provider

Generator National Stem Cell Bank (KSCB)
Owner National Institute of Health, Korea
Distributors
Derivation country South Korea

External Databases

Cellosaurus CVCL_D6MJ
Wikidata Q127382577

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA113398691

Ethics

Also have a look at the ethics information for the parental line KSCBi017-A .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Thermo Fisher
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution Research use only

hIPSC Derivation

General

The source cell information can be found in the parental cell line KSCBi017-A.
Passage number reprogrammed 5

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line KSCBi017-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 35
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
GATA binding protein 6 (target)
Gene knock-in
18q11.2
CRISPR-associated (CRISPR/Cas) System