Royan H10

General#

Cell Line

hPSCreg Name RIe010-A
Alternative name(s)
Royan H10
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Royan Institute (RI)
Derivation country Iran

External Databases

Cellosaurus CVCL_9382

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? National

hESC Derivation#

PGD Embryo?
Yes

Culture Conditions#

CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source:
Serum concentration: 20 %
Supplements
2 mM L-glutamine , 0.1 mM β-mercaptoethanol, 1% nonessential amino acids, 100 units/ml penicillin and 100 µg/ml streptomycin and insulin-transferrin-selenite

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)