Royan H10

General

Cell Line

hPSCreg name RIe010-A
Cite as:
RIe010-A (RRID:CVCL_9382)
Alternative name(s)
Royan H10
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 30th August 2022
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Provider

Generator Royan Institute (RI)
Derivation country Iran

External Databases

Cellosaurus CVCL_9382
Wikidata Q54950983

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? National
Approval number
Is there an MTA available for the cell line? Yes

hESC Derivation

PGD Embryo?
Yes

Culture Conditions

CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source:
Serum concentration: 20 %
Supplements
2 mM L-glutamine , 0.1 mM β-mercaptoethanol, 1% nonessential amino acids, 100 units/ml penicillin and 100 µg/ml streptomycin and insulin-transferrin-selenite

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)