Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background


Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies. Resource table. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wang Y, Zhang J, Zhang Y, Huang D, Zhao J, Li G, Lei Y
Journal Stem cell research
Publication Date 2017 Oct;24:33-35
PubMed 29034892
DOI 10.1016/j.scr.2017.08.008

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