Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background
Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies. Resource table. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Wang Y, Zhang J, Zhang Y, Huang D, Zhao J, Li G, Lei Y|
|Journal||Stem cell research|
|Publication Date||2017 Oct;24:33-35|