Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male
Summary
Peripheral blood was extracted from a 48-year old healthy male donor. Induced pluripotent stem cells (iPSC) were reprogrammed by sendai virus encoding Klf-4, c-Myc, Oct-4, and Sox-2. The iPSC line showed pluripotency, which was verified by immunofluorescence staining. The iPSC line showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This cell line can be served as healthy control for studying inherited disease. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Wu H, Cheng Y, Lv Z, Liu L, Gong W, Zhang S |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102221 |
| PubMed | 33677395 |
| DOI | 10.1016/j.scr.2021.102221 |