Establishment of human embryonic stem cell line Amicqui-2 using poor-quality embryos from Mexican population
Summary
Although investigation with human embryonic stem cells (HESC) is not decreasing, the derivation of new lines has been diminished. The preeminence of only a few HESC lines in research is accompanied by lack of universal applicability of results as well as by genetic under-representation. We previously reported the derivation of one line with male karyotype from Mexican population. Here, we derived one HESC line (Amicqui-2) with female karyotype from poor-quality embryos. These line comply the pluripotent requirements (normal karyotype, detection of pluripotency-associated markers, mycoplasma test and teratoma formation) and could be a valuable model for studying diseases specific to under-represented population. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ávila-González D, Martínez-Alarcón O, García-López G, Díaz-Martínez NE, Razo-Aguilera G, Valdespino-Vázquez MY, Moreno-Verduzco ER, Vega-Hernández E, Regalado-Hernández JC, De la Jara-Díaz JF, Molina-Hernández A, Flores-Herrera H, Portillo W, Díaz NF |
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| Journal | Stem cell research |
| Publication Date | 2019 Jan;34:101364 |
| PubMed | 30611019 |
| DOI | 10.1016/j.scr.2018.101364 |