ZIP13 clone 2
ZIPi013-B
General
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA1087896 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | No |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | No |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | https://www.sciencellonline.com |
| Alternatives to consent are available? | No |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | Unknown |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent pertain to a specific research project? | No |
| Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | No |
| Does consent expressly prevent development of commercial products? | No |
| Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? | No |
| Does consent expressly permit storage of donated embryo/tissue for an unlimited time? | No |
| Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? | No |
| Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | Yes |
| a non-profit company? | Yes |
| a for-profit corporation? | No |
| Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? | Yes |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? | No |
| Does consent permit access to medical records of the donor? | No |
| Does consent permit access to any other source of information about the clinical treatment or health of the donor? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Advanced Bioscience Resource Inc. |
| Approval number | not applicable |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Ethikkommission der medizinischen Fakultät der Christian-Albrechts Universität zu Kiel |
| Approval number | A145/11 |
| Do you have obligations to third parties in regard to the use of the cell line? | No |
| Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? | No |
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Addgene |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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| Source cell line name |
HDF51 Derived from same source line (potentially other lot and donor, see below):
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| Source cell type |
Any skin fibroblast that is part of some dermis.
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| Source cell type (free text) | Fibroblast from aborted fetus, commercially sourced through ScienCell Research Laboratories Inc., San Diego |
| Source cell line vendor | ScienCell Research Laboratories |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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Other |
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| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| CO2 Concentration | 5 % |
| Medium | TeSR™ E8™ |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
| Pluripotency Score | Novelty Score | |
| 21.21 | 1.32 |
Report
PluriTest ZIPi013-B.pdf
PluriTest ZIPi013-B
1-s2.0-S1873506118302472-gr2_lrg.jpg
PluriTest ZIPi013-E
Morphology pictures
ZIPi013-B.tiff
ZIPi013-B morphology (phase contrast)
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Scorecard
1-s2.0-S1873506118302472-gr2_lrg.jpg
Scorecard results for ZIPi013-B and ZIPi013-E.
Differentiation Potency
In vitro directed differentiation
Morphology
ZIPi013-B, FOXA2.tiff
FOXA2 immunofluorescence
ZIPi013-B, SOX17.tiff
SOX17 immunofluorescence
Endoderm ZIPi013-B .tiff
Endoderm morphology (phase contrast)
Protocol or reference
Definitive Endoderm differentiation protocol.pdf
iPSCs to definitive endoderm differentiation protocol
In vitro directed differentiation
| Marker | Expressed |
| Myosin heavy chain 7 |
Yes |
| actinin alpha 2 |
Yes |
Morphology
ZIPi013-B, Actinin alpha 2.tiff
Alpha actinin 2 immunofluorescence pic
ZIPi013-B, Myosin heavy chain 7.tiff
Myosin heavy chain 7 immunofluorescence pic
Cardiomyocytes ZIPi013-B.tiff
Cardiomyocytes morphology (phase contrast)
Protocol or reference
Manual_Pluricyte Cardiomyocyte Differentiation Kit-2.pdf
iPSCs to cardiomyocyte differentiation protocol
In vitro directed differentiation
Morphology
ZIPi013-B, Beta lll Tubulin.tiff
Beta lll Tubulin immunofluorescence
ZIPi013-B, MAP2.tiff
MAP2 immunofluorescence
Neurons ZIPi013-B.tiff
Neurons morphology (Phase contrast pic)
Protocol or reference
Neural Stem Cell differentiation protocol.pdf
iPSCs to Neural Stem Cell differentiation protocol
Neurons differentiation protocol.pdf
Neural Stem Cells to terminally differentiatied neuron protocol
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
ZIPi013-B and ZIPi013-E: 46XX, 450–500 bphs (band per haploid set)
Passage number: 14
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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