hESBGN-03 (BG03)

General

Cell Line

hPSCreg Name
VIACe003-A
Alternative name(s)
hESBGN-03 (BG03)
Cell line type Human embryonic stem cell (hESC)
Last update 3rd September 2022
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Provider

Generator Novocell, Inc. (VIAC)
Derivation country United States

External Databases

Cellosaurus CVCL_9730
CLO CLO_0100190
Wikidata Q54796226

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology C
Stephenson 2007
Trophectoderm morphology g
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
SSEA-1
No
Differentiation Potency
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 17

Other Genotyping (Cell Line)