SH38
SSMUe008-A
General
Cell Line |
|
| hPSCreg name | SSMUe008-A |
| Cite as: | SSMUe008-A (RRID:CVCL_C729) |
| Alternative name(s) |
SH38
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 30th August 2022 |
| User feedback | |
Provider |
|
| Generator |
Shanghai Second Medical University (SSMU)
Contact:
Center for Developmental Biology |
| Derivation country | China |
External Databases |
|
| Cellosaurus | CVCL_C729 |
| Wikidata | Q54955710 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cells Cellfinder Ont Id: EFO_0004040 |
||||||
| Passage method | Enzymatically | ||||||
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
|
|||
| ZFP42 (REX-1) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| Alkaline Phosphatase |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.