The cell line is not validated yet.

General#

Cell Line

hPSCreg Name UJSi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 17th February 2019
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Provider

Generator Jiangsu University (UJS)
Derivation country China

External Databases

BioSamples SAMEA5573422

General Information

Is the cell line available in principle? Available with restrictions: Available for clinical research

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Schizophrenia
The donor is affected.
Synonyms
  • Schizophrenia
  • schizophrenia

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5573423

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Alternatives to consent are available? No
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Policy for access to genetic information Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? The Ethics Committee of Bio-X Institutes in Shanghai Jiao Tong University
Approval number M16035
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? The Ethics Committee of Bio-X Institutes in Shanghai Jiao Tong University
Approval number M16035
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell line name 22C FIBROBLAST
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Methods used
Immunostaining, PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
Marker Present Absent
mCpG
OCT4 X
Self-renewal
Unkown
Endoderm
Unkown
Mesoderm
Unkown
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46.xx

Other Genotyping (Cell Line)