The cell line is not validated yet.

General#

Cell Line

hPSCreg Name UJSi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 17th February 2019
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Provider

Generator Jiangsu University (UJS)
Derivation country China

External Databases

BioSamples SAMEA5573422

General Information

Is the cell line available in principle? Available with restrictions: Available for clinical research

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Schizophrenia
The donor is affected.
Synonyms
  • Schizophrenia
  • schizophrenia

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA5573423

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name 22C FIBROBLAST
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Methods used
Immunostaining, PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Collagenase
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
Marker Present Absent
mCpG
OCT4 X
Self-renewal
Unkown
Endoderm
Unkown
Mesoderm
Unkown
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46.xx

Other Genotyping (Cell Line)