The cell line is not validated yet.

General

Cell Line

hPSCreg name MLUi002-G
Cite as:
MLUi002-G
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MLUi001-M
Donor diseases:
Schizophrenia
NMIi002-A
Donor diseases:
Schizophrenia
VUi005-A
(787.3)
Donor diseases:
Schizophrenia
VUi006-A
(SCZ 21.5)
Donor diseases:
Schizophrenia
NMIi005-A
Donor diseases:
Schizophrenia
VUi008-A
(SCZ 6.2)
Donor diseases:
Schizophrenia
VUi009-A
(SCZ 10.1)
Donor diseases:
Schizophrenia
VUi010-A
(SCZ 1.1)
Donor diseases:
Schizophrenia
VUi011-A
(SCZ 3.5)
Donor diseases:
Schizophrenia
VUi012-A
(SCZ 7.2)
Donor diseases:
Schizophrenia
VUi013-A
(804.3)
Donor diseases:
Schizophrenia
NMIi002-B
Donor diseases:
Schizophrenia
VUi017-A
(SCZ4.3)
Donor diseases:
Schizophrenia
VUi024-A
(SCZ 9.4)
Donor diseases:
Schizophrenia
MHRCCGi001-A
(P1SH)
Donor diseases:
Schizophrenia
NMIi004-A
Donor diseases:
Schizophrenia
NMIi006-A
Donor diseases:
Schizophrenia
SZGJMSi001-A
(ZLP-2)
Donor diseases:
Schizophrenia
NMIi006-B
Donor diseases:
Schizophrenia
WCHi006-A
Donor diseases:
Schizophrenia
WCHi003-A
Donor diseases:
Schizophrenia
UJSi001-A
Donor diseases:
Schizophrenia
Last update 30th January 2024
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Medical Faculty of the Martin Luther University Halle-Wittenberg (MLU)
Derivation country Germany

External Databases

BioSamples SAMEA115162595

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 20-24
Ethnicity European

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Schizophrenia
  • schizophrenia
  • Schizophrenia, unspecified
Genetic variants
2p16.3 (NRXN1)

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA115162596

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Prof. Dr. med. Dan Rujescu, Klinische Abteilung für Allgemeine Psychiatrie an der Universitätsklinik für Psychiatrie und Psychotherapie (UKPP), Medizinische Universität Wien und AKH Wien
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Prof. Dr. med. Dan Rujescu, Klinische Abteilung für Allgemeine Psychiatrie an der Universitätsklinik für Psychiatrie und Psychotherapie (UKPP), Medizinische Universität Wien und AKH Wien
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution

hIPSC Derivation

General

Source cell type
A biospecimen consisting of lymphocytes that have been isolated from whole blood and are then infected in vitro by Epstein-Barr virus (EBV; HHV4). The infected cells are then cultured to select indefinitely proliferating colonies that can be maintained in tissue culture.
Synonyms
  • EBV Immortalized Lymphocytes
Age of donor (at collection) 20-24

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzymatically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
SSEA-1
No
Alkaline phosphatase staining
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
CXCR4
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
TBXT
Yes
MEOX1
Yes
PRRX1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
MSI1
Yes
SHH
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)