RCi89, Thp3C-9

General#

Cell Line

hPSCreg Name RCi001-B
Alternative name(s)
RCi89, Thp3C-9
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 13th December 2017
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Provider

Generator Pfizer Limited - Pfizer (PFIZ)
Distributors

External Databases

BioSamples SAMEA3106040
Cellosaurus CVCL_9S42
ECACC 66540048
EBiSC RCi001-B

General Information

Publications
Projects
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Erythromelalgia
The donor is affected.
Genetic variants
SCN9A (target)
2q24.3
Heterozygous
In the publication PMID:27099175, heterozygous point mutations were confirmed by Sanger sequencing: V400M: GTG(V) >>ATG(M)

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105813

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
erythroblast
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Synonyms
  • normoblast
Source cell type (free text) Blood - PBMC erythroblasts
Passage number reprogrammed NA

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY[14]/47,XY,+12[5]
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes