SFC813-03-07

The cell line is not validated yet.

General

Cell Line

hPSCreg name STBCi035-C
Cite as:
STBCi035-C (RRID:CVCL_RC10)
Alternative name(s)
SFC813-03-07
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi035-A
(SFC813-03-09)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi035-B
(SFC813-03-03)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-A
(SFC811-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-B
(SFC811-03-04)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi017-C
(SFC811-03-05)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi036-A
(SFC814-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
STBCi129-A
(SFC137-03-01)
Donor's gene variants:
SCN9A
Donor diseases:
neuropathy
KAIMRCi003-A
(DRVT-iPSC#1)
Donor's gene variants:
SCN9A, CPLX1
Donor diseases:
Dravet Syndrome
KAIMRCi003-B
(DRVT-iPSC#2)
Donor's gene variants:
SCN9A, CPLX1
Donor diseases:
Dravet Syndrome
PFIZi001-A
(EM1-3F)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi002-A
(EM1-7M)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
RCi002-A
(FP5C-14, RCi92)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi002-B
(FP5C-16, RCi93)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi001-A
(Thp3C-6, RCi88)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
RCi001-B
(RCi89, Thp3C-9)
Donor's gene variants:
SCN9A, SCN9A
Donor diseases:
Primary erythromelalgia
STBCi139-A
(SFC101-03-01)
Donor diseases:
neuropathy
STBCi144-A
(SFC123-03-01)
Donor diseases:
neuropathy
PFIZi003-A
(EM2-7M)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
PFIZi004-A
(EM2-7F)
Donor's gene variants:
SCN9A
Donor diseases:
Primary erythromelalgia
STBCi049-A
(SFC040-03-02)
Donor diseases:
neuropathy
STBCi049-B
(SFC040-03-03)
Donor diseases:
neuropathy
STBCi050-A
(SFC041-04-01)
Donor diseases:
neuropathy
STBCi050-B
(SFC041-04-02)
Donor diseases:
neuropathy
STBCi049-C
(SFC040-03-01)
Donor diseases:
neuropathy
STBCi050-C
(SFC041-04-03)
Donor diseases:
neuropathy
STBCi181-A
(SFC247-03-01)
Donor diseases:
neuropathy
STBCi183-A
(SFC250-03-01)
Donor diseases:
neuropathy
STBCi185-A
(SFC253-03-01)
Donor diseases:
neuropathy
STBCi195-A
(SFC286-03-01)
Donor diseases:
neuropathy
STBCi196-A
(SFC287-03-01)
Donor diseases:
neuropathy
STBCi197-A
(SFC288-03-01)
Donor diseases:
neuropathy
STBCi223-A
(SFC363-04-01)
Donor diseases:
neuropathy
STBCi226-A
(SFC366-03-01)
Donor diseases:
neuropathy
STBCi228-A
(SFC368-03-01)
Donor diseases:
neuropathy
STBCi104-A
(SFC836-03-01)
Donor diseases:
neuropathy
STBCi230-A
(SFC372-03-01)
Donor diseases:
neuropathy
STBCi231-A
(SFC374-03-01)
Donor diseases:
neuropathy
STBCi037-A
(SFC816-03-02)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi037-B
(SFC816-03-08)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi037-C
(SFC816-03-13)
Donor's gene variants:
GARS1
Donor diseases:
neuropathy
STBCi124-A
(SFC135-03-01)
Donor diseases:
neuropathy
STBCi140-A
(SFC102-03-01)
Donor diseases:
neuropathy
STBCi059-A
(SFC015-01-05)
Donor diseases:
neuropathy
STBCi059-B
(SFC015-01-06)
Donor diseases:
neuropathy
STBCi059-C
(SFC015-01-10)
Donor diseases:
neuropathy
STBCi060-A
(SFC038-07-05)
Donor diseases:
neuropathy
STBCi060-B
(SFC038-07-06)
Donor diseases:
neuropathy
STBCi060-C
(SFC038-07-07)
Donor diseases:
neuropathy
STBCi182-A
(SFC248-03-01)
Donor diseases:
neuropathy
STBCi184-A
(SFC251-03-01)
Donor diseases:
neuropathy
Last update 30th May 2023
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Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA104493870
EBiSC STBCi035-C
Cellosaurus CVCL_RC10
Wikidata Q54956450

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 35-39

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Genetic variants
SCN9A (target)
SCN9A severe inherited erythromelalgia - Heterozygous missense mutation (a C to T substitution at 2572) resulting in the substitution of leucine 858 with phenylalanine L858F

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493688

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information zameel.cader@ndcn.ox.ac.uk
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? NHS-NRES Committee
Approval number 12/LO/0017
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? NHS-NRES Committee
Approval number 12/LO/0017
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: Only to be used into research into Painful Channelopathies / Pain syndromes. Material shall not be sold, transplanted into any human being or used to create egg or sperm cells (gametes) or embryos. The material shall not be used for direct exploitation. For the purposes of this, Direct exploitation means to develop for commericalization or to commercialize the Material.
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 35-39

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)