SFC813-03-03

General#

Cell Line

hPSCreg Name STBCi035-B
Alternative name(s)
SFC813-03-03
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA104493869
EBiSC STBCi035-B
ECACC 66540718
Cellosaurus CVCL_RC09

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
neuropathy (primary disease)
Genetic variants
SCN9A severe inherited erythromelalgia - Heterozygous missense mutation (a C to T substitution at 2572) resulting in the substitution of leucine 858 with phenylalanine L858F

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493688

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

No characterisation data could be found.

Characterisation#

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)