EM1-7M
PFIZi002-A
General#
Cell Line |
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hPSCreg Name | PFIZi002-A |
Alternative name(s) |
EM1-7M
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator | Pfizer Limited - Pfizer (PFIZ) |
External Databases |
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Cellosaurus | CVCL_9S32 |
General Information |
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Publications | |
Is the cell line available in principle? |
Yes |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
erythroblastA nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Synonyms
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Source cell origin |
bloodA fluid that is composed of blood plasma and erythrocytes.
Synonyms
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Passage number reprogrammed | 10 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Vector free reprogramming |
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Other |
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Selection criteria for clones | morphology, pluripotency gene expression, differentiation into sensory neurons |
Derived under xeno-free conditions |
Unkown |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
Dispase
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O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium | mTeSR™ 1 |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46, XX
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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