SFC811-03-05

General#

Cell Line

hPSCreg Name STBCi017-C
Alternative name(s)
SFC811-03-05
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA104493632
EBiSC STBCi017-C
ECACC 66540672
Cellosaurus CVCL_RB67

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
neuropathy (primary disease)
Genetic variants
SCN9A heterozygotes for a) Premature stop codon in coding exon 15 p.R830X and b) A 1 bp deletion within coding exon 26 which induces a frameshift at position 1773 in the C terminal domain of the channel c.2488C>T

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493630

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

No characterisation data could be found.

Characterisation#

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)