Generation of a genetically-modified induced pluripotent stem cell line harboring an oncogenic gene variant KRAS p.G12V


Activating KRAS codon 12 gene variants are known to cause severe RAS-MAPK and PI3K-AKT signaling pathway hyperactivity and are frequently involved in the development of various carcinomas. Here, we describe the generation of a human iPSC line harboring the common oncogenic KRAS p.G12V variant by using CRISPR/Cas9 technology. The established KRASG12V iPSC line allows the study of oncogenic KRAS-induced signaling dysregulation and its impact on cell physiology in various iPSC-derived cell types and tissues. Furthermore, it might serve as a powerful platform for drug and toxicity screenings to identify new chemotherapeutic drugs. Copyright © 2023. Published by Elsevier B.V.

Authors Busley AV, Kleinsorge M, Cyganek L
Journal Stem cell research
Publication Date 2023 Jun;69:103105
PubMed 37121193
DOI 10.1016/j.scr.2023.103105

Research Projects

Cell Lines