A human ESC line for efficient CRISPR editing of pluripotent stem cells


Human pluripotent stem cells (hPSC) can be directed to differentiate in vitro into insulin-prorducing beta cells (SC-β). Although these cells accurately respond to glucose and can reverse diabetes in preclinical models improvments in the final cell products are desirable. For example, safety, controlling the cellular compositions and protection against immune rejection may be addressed by genetic modifications of SC-β pre-transplantation. To screen for gene targets, we have generated a human embryonic stem cell line (hESC) that constitutively express the enhanced specificity Streptococcus pyogenes Cas9 (eSpCas9) gene, knocked-in into the GAPDH locus. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Sintov E, Gerace D, Melton DA
Journal Stem cell research
Publication Date 2021 Dec;57:102591
PubMed 34741877
DOI 10.1016/j.scr.2021.102591

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