SYSU-2

General

Cell Line

hPSCreg name SYSUe002-A
Cite as:
SYSUe002-A (RRID:CVCL_C068)
Alternative name(s)
SYSU-2
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Sun Yat-sen University (SYSU)
Derivation country China

External Databases

Cellosaurus CVCL_C068
Wikidata Q54971288

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Cimmittee of Sun Yat-sen University

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery

Culture Conditions

Feeder cells Mouse Embryonic Fibroblasts
Cellfinder Ont Id: EFO_0004040
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
Alkaline Phosphatase
Yes
SSEA-1
No
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 18

Other Genotyping (Cell Line)