BFC4-C, BFC4-CRISPR-corrected
CIAUi003-A-1
General
Cell Line |
|
| hPSCreg name | CIAUi003-A-1 |
| Cite as: | CIAUi003-A-1 (RRID:CVCL_C6P2) |
| Alternative name(s) |
BFC4-C, BFC4-CRISPR-corrected
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
CIAUi003-A (MCCIi005-A-1, BFC4) Donor's gene variants: CASQ2, CASQ2 Donor diseases: catecholaminergic polymorphic ventricular tachycardia 2 |
| Last update | 20th January 2023 |
| User feedback | |
Provider |
|
| Generator | Centenary Institute of Cancer Medicine and Cell Biology (CIAU) |
| Owner | Centenary Institute of Cancer Medicine and Cell Biology (CIAU) |
| Distributors | |
| Derivation country | Australia |
External Databases |
|
| BioSamples | SAMEA112186035 |
| Cellosaurus | CVCL_C6P2 |
| Wikidata | Q117704246 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | North-west European |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA112190001 |
Ethics
Also have a look at the ethics information for the parental line
CIAUi003-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
CIAUi003-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Notes on reprogramming vector detection | No vectors detected |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
|
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