MCCIi005-A-1, BFC4
CIAUi003-A
General
Cell Line |
|
| hPSCreg name | CIAUi003-A |
| Cite as: | CIAUi003-A (RRID:CVCL_WM06) |
| Alternative name(s) |
MCCIi005-A-1, BFC4
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
CIAUi003-A-1 (BFC4-C, BFC4-CRISPR-corrected) Donor's gene variants: CASQ2, CASQ2 Donor diseases: catecholaminergic polymorphic ventricular tachycardia 2 |
| Last update | 20th January 2023 |
| User feedback | |
Provider |
|
| Generator | Centenary Institute of Cancer Medicine and Cell Biology (CIAU) |
| Owner | Centenary Institute of Cancer Medicine and Cell Biology (CIAU) |
| Distributors | |
| Derivation country | Australia |
External Databases |
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| BioSamples | SAMEA112186036 |
| Cellosaurus | CVCL_WM06 |
| Wikidata | Q95985819 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclones | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | North-west European |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA112190001 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | c.semsarian@centenary.org.au |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethics Review Committee, Sydney Local Health District |
| Approval number | X19-0108 and 2019/ETH00461 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
|
| Source cell origin |
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Notes on reprogramming vector detection | No vectors detected |
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ Plus
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Normal
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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