Generation of iPSC from fetal fibroblast cells obtained from an abortus with type-I tri-allelic variants

Summary

An integration free iPSC line was generated from fibroblast obtained from the skin of an aborted fetus in feeder free conditions using episomal based vectors expressing the pluripotency factors. The cell line generated was characterized and tested for pluripotency both in vitro and in vivo by teratoma formation and differentiation into defined lineages and brain organoids. Cell line reported here is shown to be mycoplasma free. Copyright © 2020 CSIR Centre for Cellular and Molecular biology. Published by Elsevier B.V. All rights reserved.

Authors Jamwal VS, Vishnu VV, Domreddy A, Parekh Y, Kumar BK, Chandra Shekar P, Singh S
Journal Stem cell research
Publication Date 2020 Aug 27;48:101963
PubMed 32916634
DOI 10.1016/j.scr.2020.101963

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