SHCDNi006-A

SHCDN006

General#

Cell Line

hPSCreg Name
SHCDNi006-A
Alternative name(s)
SHCDN006
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 6th May 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Shanghai Children's Hospital (SHCDN)
Owner Department of Neurology (BCHNEU)
Distributors

External Databases

BioSamples SAMEA14207087

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Additional restrictions:

none

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
epilepsy
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • epilepsy
  • Epileptic attack, NOS
  • Epilepsy, unspecified, with intractable epilepsy
  • Generalized seizure (finding)
  • Epileptic seizures
  • Other forms of epilepsy (disorder)
  • Epileptic seizures, NOS
  • [X]Other epilepsy (disorder)
  • Seizure disorder (disorder)
  • Generalized fit
  • Epilepsy (disorder)
  • Epileptic fits
  • Seizure disorder
  • Other forms of epilepsy
  • Epilectic attack, NOS
  • Other forms of epilepsy, with intractable epilepsy
  • Epileptic fits, NOS
  • Epileptic disorder
  • Epilepsy, NOS
  • EPILEP NEC W/O INTR EPIL
  • EPILEPSY NOS W INTR EPIL
  • Epileptic Seizure
  • Epileptic attack
  • Epileptic seizure (finding)
  • seizure disorder
  • [X]Other epilepsy
  • EP - Epilepsy
  • EPILEPSY NEC W INTR EPIL
  • Other forms of epilepsy NOS
  • Other forms of epilepsy and recurrent seizures
  • Epileptic convulsions, NOS
  • Epileptic disorder, NOS
  • Epilepsy, unspecified, without mention of intractable epilepsy
  • Epilepsy, unspecified
  • Epilepsy and recurrent seizures
  • Epilepsy NOS (disorder)
  • EPILEP NOS W/O INTR EPIL
  • Epileptic fit
  • Epilepsy NOS
  • Other forms of epilepsy, without mention of intractable epilepsy
  • Epileptic
  • Generalized seizure
  • Generalized convulsion
  • Generalised seizure
  • Generalised convulsion
  • Other forms of epilepsy NOS (disorder)
  • EF - Epileptic fit
  • Generalised fit
  • Epileptic convulsions
show more synonyms
Genetic variants
Chromosome 9
NM_020822.2 c.2800 G>A
NP_065873.2 p.Ala934Thr
Heterozygous
Disease associated phenotypes
  • a five-month-old Han Chinese girl presented with EIMFS at 42-day-old whose convulsion was manifested as staring with eyes to one side (left or right), accompanied by asymmetric shaking of lower limbs, no lip blue, no foaming in the mouth, lasting for about 2 minutes.
Family history NONE

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
The karyotype of SHCDNi006-A iPSCs was examined by Giemsa-banding to verify genetic stability, and showed that 23 pairs of chromosomes with normal structure
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA14207088

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent permit unforeseen future research, without further consent? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Shanghai Children's Hospital
Approval number REK 2017R021-F01
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee of Shanghai Children's Hospital
Approval number REK 2017R021-F01
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms
Source cell origin
Blood
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms
Source cell line vendor Shanghai Children's Hospital
Passage number reprogrammed 10

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
Protein

Other

Selection criteria for clones cells are small and present a compact group
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method ACCUTASE
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
PAX6
Yes
AFP
Yes
Brachyury
Yes
SSEA-4
Yes
OCT4
Yes
Nanog
Yes
Marker Present Absent
mCpG
OCT4 X
Report
Morphology pictures
To verify the tri-lineage differentiation potential of the cells, the markers SOX1 (for ectoderm), BMP4 (for mesoderm) and GATA4 (for endoderm) were used clarify differentiation capacity by Real-time qPCR experiment. The relative fold changes of gene expression between differentiated cells and iPSCs. (Ectoderm: SOX1; Mesoderm: BMP4; Endoderm: GATA4), n=3.
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
GATA4
Unknown
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
BMP4
Unknown
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
SOX1
Unknown

Microbiology / Virus Screening

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
The karyotype of SHCDNi006-A iPSCs was examined by Giemsa-banding to verify genetic stability, and showed that 23 pairs of chromosomes with normal structure
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes