PC117_c2

General

Cell Line

hPSCreg name INSRMi002-A
Cite as:
INSRMi002-A (RRID:CVCL_WN90)
Alternative name(s)
PC117_c2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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(PC131_c8)
Donor's gene variants:
DEPDC5
Donor diseases:
epilepsy
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Donor's gene variants:
SCN1A
Donor diseases:
epilepsy
YSCHi001-A
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Donor diseases:
epilepsy
PFIZi024-A
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Donor's gene variants:
GABRA1
Donor diseases:
epilepsy
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Donor diseases:
epilepsy
PFIZi025-A
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Donor's gene variants:
KCNC1
Donor diseases:
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Donor diseases:
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INSRMi003-A
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Last update 27th July 2024
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Provider

Generator INSERM (INSRM)
Contact:
INSERM (INSRM)
Owner INSERM (INSRM)
Distributors
Derivation country France

External Databases

BioSamples SAMEA5794618
Cellosaurus CVCL_WN90
Wikidata Q94318176

General Information

Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 30-34
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Stage
epilepsy
Synonyms
  • epilepsy
  • Epileptic attack, NOS
  • Epilepsy, unspecified, with intractable epilepsy
  • Generalized seizure (finding)
  • Epileptic seizures
  • Other forms of epilepsy (disorder)
  • Epileptic seizures, NOS
  • [X]Other epilepsy (disorder)
  • Seizure disorder (disorder)
  • Generalized fit
  • Epilepsy (disorder)
  • Epileptic fits
  • Seizure disorder
  • Other forms of epilepsy
  • Epilectic attack, NOS
  • Other forms of epilepsy, with intractable epilepsy
  • Epileptic fits, NOS
  • Epileptic disorder
  • Epilepsy, NOS
  • EPILEP NEC W/O INTR EPIL
  • EPILEPSY NOS W INTR EPIL
  • Epileptic Seizure
  • Epileptic attack
  • Epileptic seizure (finding)
  • seizure disorder
  • [X]Other epilepsy
  • EP - Epilepsy
  • EPILEPSY NEC W INTR EPIL
  • Other forms of epilepsy NOS
  • Other forms of epilepsy and recurrent seizures
  • Epileptic convulsions, NOS
  • Epileptic disorder, NOS
  • Epilepsy, unspecified, without mention of intractable epilepsy
  • Epilepsy, unspecified
  • Epilepsy and recurrent seizures
  • Epilepsy NOS (disorder)
  • EPILEP NOS W/O INTR EPIL
  • Epileptic fit
  • Epilepsy NOS
  • Other forms of epilepsy, without mention of intractable epilepsy
  • Epileptic
  • Generalized seizure
  • Generalized convulsion
  • Generalised seizure
  • Generalised convulsion
  • Other forms of epilepsy NOS (disorder)
  • EF - Epileptic fit
  • Generalised fit
  • Epileptic convulsions
show more synonyms
Genetic variants
DEPDC5 (target)
22q12
NM_001242896: c.4260delG
NP_001229825: : p.Glu1420Argfs*153
Heterozygous
PMID: 27173016
Family history yes
Is the medical history available upon request? no
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

All cell lines of this donor's relatives
Has brother:

External Databases (Donor)

BioSamples SAMEA5795689

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? comité de protection des personnes, CPP Ile de France I
Approval number C 14-56
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? comité de protection des personnes, CPP Ile de France I
Approval number C 14-56
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
The fluid portion of the blood in which the particulate components are suspended.
Synonyms
  • plasma
Source cell origin
The fluid portion of the blood in which the particulate components are suspended.
Synonyms
  • plasma
Age of donor (at collection) 30-34
Collected in 2017

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Unknown
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Laminin
Feeder cells
No
Passage method Enzymatically
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Morphology
Mesoderm
Ont Id: UBERON_0000926
Mesoderm
Ont Id: UBERON_0000926
Ectoderm
Ont Id: UBERON_0000924
Ectoderm
Ont Id: UBERON_0000924

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY Normal karyotype
Passage number: 10
Karyotyping method: R-banding karyotyping (RHG, resolution 400-500 bands), 10 metaphasis

Other Genotyping (Cell Line)